| Autor: |
Mandelkow, Eckhard, Dewar, Michael J. S., Dunitz, Jack D., Hafner, Klaus, Heilbronner, Edgar, Itô, Shô, Lehn, Jean-Marie, Niedenzu, Kurt, Raymond, Kenneth N., Rees, Charles W., Vögtle, Fritz, Bazin, D., Benfatto, M., Bianconi, A., Clement, R., Dexpert, H., Galy, J., Garcia, J., Lagarde, P., Laggner, P. |
| Zdroj: |
Synchrotron Radiation in Chemistry & Biology I; 1988, p203-232, 30p |
| Abstrakt: |
Small angle X-ray scattering and diffraction methods can provide information about chromatin structure at the levels of nucleosomes, chromatin fibre and whole nuclei. Making use of the high flux and point focusing achieved on synchrotron radiation cameras it has been possible to carry out systematic static and kinetic investigations of chromatin structure with minimum damage or perturbation on fresh samples. The results provide a strong evidence for a preformed 3-D zigzag structure at low ionic strength which folds instantaneously into the "30 nm filament" upon increasing the ionic strength. The extent of folding at a given ionic strength depends on the nature of the cations and can be influenced by other substances which bind to DNA. Models of chromatin fibre structure are discussed in the context of various experimental results. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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