| Autor: |
Smith, Rodney, Schomberg, Michael, Hakemeyer, Christian, Büntemeyer, Heino, Stiens, Lars, Lehmann, Jürgen |
| Zdroj: |
Cell Technology for Cell Products; 2007, p473-477, 5p |
| Abstrakt: |
Animal derived protein hydrolysates are commonly used as a complex supplement for cell culture media because of their well known growth promoting properties. In an approach to find components being responsible for this effect we fractionated a protein hydrolysate by solid phase extraction (SPE) on a C18-matrix. After lyophilization and further preparation the fractions were tested for their proliferation enhancing ability with a hybridoma cell line in a serum-free DMEM/F12 medium supplemented with human serum albumin and transferrin. We could demonstrate a growth promoting effect caused by a rather hydrophilic fraction. In further investigations preparative High Performance Liquid Chromatography (HPLC) was used to produce growth promoting fractions of less complexity for cell evaluation and analytical HPLC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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