| Autor: |
Smith, Rodney, Macke, L., Wrobel, B., Meyring, W., Garritsen, H.S.P., Wörmann, B., Rhode, M., Lindenmaier, W., Dittmar, K.E.J. |
| Zdroj: |
Cell Technology for Cell Products; 2007, p239-242, 4p |
| Abstrakt: |
Dendritic cells (DC) represent the antigen-presenting cells which play a decisive role in the regulation of immune responses. Large numbers of monocyte-derived DC can be generated from leukapheresis cells for clinical applications by positive selection of CD14+ cells or by negative selection (removal of CD2+ and CD19+ cells). The positive selection showed a lower yield but higher purity of monocytes. Additionally, we compared the culture flask system (as "gold standard„) with our integrated-closed-bag system designed for clinical use. Bags resulted in slightly lower yields of viable cells on day 7 compared to flasks. Structural and functional properties of DC generated by these cultivation conditions showed no significant difference. A high donor variability was observed. In regard to clinical application and GMP compliance CD14+ selection in combination with our closed-bag system is a promising approach to generate defined dendritic cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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