| Autor: |
Walker, John M., Clegg, Roger A., Pryde, James G. |
| Zdroj: |
Protein Targeting Protocols (9780896034877); 1998, p23-33, 11p |
| Abstrakt: |
Cultured mammalian cells and tissues can be solubilized by the nonionic detergent Triton X-114 (octylphenoxy polyethoxyethanol). This detergent, when in solution above its critical micelle concentration, increases its micelle weight when warmed from 0 to 20°C and in the process decreases its critical micelle concentration. This induces intermicellar interactions, which lead to turbidity (the cloud point) and phase separation of the detergent at 20°C. A simple low-speed centrifugation step recovers the detergent-enriched phase as an oily pellet. Following solubilization and warming to 20°C, integral membrane proteins partition into the detergent-enriched phase, and peripheral and cytosolic proteins are recovered from the detergent-depleted aqueous phase. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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