Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids.

Autor: Walker, John M., Loyola-Vargas, Victor M., Hernández-Domínguez, Elizabeta, Campos-Tamayo, Freddy, Carrillo-Pech, Mildred, Vázquez-Flota, Felipe
Zdroj: Plant Cell Culture Protocols (9781588295477); 2006, p349-355, 7p
Abstrakt: A protocol for the establishment of in vitro shoot cultures of Catharanthus roseus is described. Shoots can be maintained for more than 1 yr without evidence of tissue vitrification, disaggregation, or callus formation. Vindoline was the main alkaloid accumulated, reaching values similar to those found in leaves from field-grown plants, after a long period of culture. An induction methodology to reduce such waiting time is also presented. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index