Isolation and Culture of Human Osteoblasts Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran,.

Autor: Walker, John M., Picot, Joanna, Gartland, Alison, Buckley, Katherine A., Dillon, Jane P., Curran, Judith M., Hunt, John A., Gallagher, James A.
Zdroj: Human Cell Culture Protocols (9781588292223); 2005, p29-54, 26p
Abstrakt: Bone is a complex tissue that contains at least four different cell types of the osteoblastic lineage. (1) Active osteoblast-a plump, polarized, cuboidal cell rich in organelles involved in the synthesis and secretion of matrix proteins. (2) Osteocyte-an osteoblast with low metabolic activity that has been engulfed in matrix during bone formation and entombed in lacunae. (3) Bone-lining cell-osteoblasts that have avoided entombment in lacunae and lose their prominent synthetic function; these cells cover most of the bone surfaces in mature bone. (4) Preosteoblast-a fibroblastic proliferative cell with osteogenic capacity. In addition, bone contains cells of a distinct lineage, the osteoclast (reviewed in Ch. 4). [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index