Chloramphenicol Acetyl Transferase Assay.

Autor: Walker, John M., Jones, Heddwyn, Davey, Michael R., Blackhall, Nigel W., Power, J. Brian
Zdroj: Plant Gene Transfer & Expression Protocols; 1996, p143-148, 6p
Abstrakt: The chloramphenicol acetyltransferase (cat) gene, isolated from transposon Tn9 of Escherichia coli (1), is a convenient genetic marker for studies of transformation. (For a detailed description of the CAT gene, seeChapter 1.) In plants, the CAT system is generally used in transient, rather than in stable, gene expression studies. Gene products are detected by assaying crude protein extracts of putatively transformed cells, usually within a few hours of transformation. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index