| Autor: |
Walker, John M., Zigova, Tanja, Sanberg, Paul R., Sanchez-Ramos, Juan R., Whittemore, Scott R., Ping Zhang, Y., Shields, Christopher B., Morassutti, Dante J., Magnuson, David S. K. |
| Zdroj: |
Neural Stem Cells: Methods & Protocols; 2002, p319-324, 6p |
| Abstrakt: |
The potential of stem cells to be used for repair of the injured or degenerating CNS is unlimited because of their capacity to differentiate into neurons, astrocytes, and/or oligodendrocytes. In addition, the fact that stem cells can be expanded in vitro prior to engrafting means that large numbers of clonally derived cells can be obtained. Finally, proliferating stem cells readily incorporate exogenous genes after transfection with eukaryotic expression vectors or infection with viral vectors, enabling their use for ex vivo gene delivery. However, the very process of engrafting into the CNS itself creates damage that can be detrimental to the survival of the engrafted cells, which is especially true when grafting into very small structures such as the spinal cord. Thus, the potential therapeutic efficacy of stem cell grafts will depend on grafting methods that both optimize graft survival and minimize the extent of the graft-induced lesion. Both of these variables are dependent on the type of system used to deliver the cells. We have compared a number of different configurations and sizes of micropipets for grafting into the adult rat spinal cord, and discuss our approaches to optimize the survival and integration of these grafts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
