| Autor: |
Mozafari, M. Reza, Paradissis, Agnes, Hatziantoniou, Sophia, Georgopoulos, Aristidis, Dimas, Konstantinos, Demetzos, Costas |
| Zdroj: |
Nanomaterials & Nanosystems for Biomedical Applications; 2007, p125-133, 9p |
| Abstrakt: |
The aim of this study was to investigate the uptake of free and liposomal sclareol and its effect on the growth inhibiting activity against MCF-7 and H-460 human cancer cell lines in vitro. Liposomes composed of EPC/DPPG at molar ratio 9:0.1, used to incorporate sclareol, were prepared by the thin-film hydration method followed by sonication. The final liposomal preparation (EPC/DPPG/Sclareol 9:0.1:5 molar ratio) as well as free sclareol (100μM) were incubated up to 96 hours with both cell lines. Sclareol was extracted from cells using the Bligh-Dyer method and was measured by HPTLC/FID. The results showed that the uptake of free sclareol by both cell lines was faster and higher compared to that of its liposomal form. In both cell lines, free sclareol showed high cytotoxicity, while the liposomal sclareol showed reduced cytotoxicity without affecting its ability to reduce the cell growth rate. These findings suggest that liposomal sclareol may possess chemotherapeutic advantages over its free form and can be used for future in vivo experiments for the treatment of these two types of human cancer [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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