| Autor: |
Walker, John M., Sun, Zhongjie, Zeineddine, Dana, Papadimou, Evangelia, Mery, Annabelle, Mènard, Claudine, Puc, Michel |
| Zdroj: |
Molecular Cardiology; 2005, p175-182, 8p |
| Abstrakt: |
Embryonic stem (ES) cells represent a source for cell-based regenerative therapies of heart failure. The pluripotency and the plasticity of ES cells allow them to be committed to a cardiac lineage following treatment with growth factors of the transforming growth factor (TGF)-β superfamily. We describe a protocol designed to turn on expression of cardiac-specific genes in undiffer entiated murine ES cells stimulated with BMP2 and/or TGF-β. Cell commit ment results in a significant improvement in spontaneous cardiac differentiation of ES cells both in vitro and in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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