| Autor: |
Walker, John M., Howlett, Anthony, Rosfjord, Edward C., Dickson, Robert B. |
| Zdroj: |
Integrin Protocols; 1999, p79-90, 12p |
| Abstrakt: |
Flow cytometry is the rapid analysis of populations of cells on a cell-by-cell basis by passing them individually through a beam of laser light, and the measurement of light emitted from the cells by scatter or fluorescence (1,2). Integrins have been studied by flow cytometry since the mid-1980s when immunologists started examining CD11 (integrins αM, αX, and αL) as markers of T-cell differentiation in mixed cell populations (3,4). These analyses highlight many of the advantages and utility of flow cytometry over other forms of integrin analysis. With the appropriate antibody, flow cytometry can identify individual cells in a mixed cell population. Cell-surface expression of integrins can be differentiated from intracellular pools by flow cytometry analysis, unlike Western blotting or immunoprecipitation. Multicolor analysis can simultaneously measure the levels of two or three different integrins, enabling the researcher to determine that both an α and β subunits are present on the plasma membrane. Lastly, flow cytometry can sterilely sort viable cells on the basis of their cell-surface integrin expression and retain their viability for further growth and analysis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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