Homogeneous Techniques for Monitoring Receptor-Ligand Interactions.

Autor: Walker, John M., Janzen, William P., Marks, James S., Burdette, Douglas S., Giegel, David A.
Zdroj: High Throughput Screening; 2002, p51-63, 13p
Abstrakt: Radioligand binding is a well-established technique that has been used to study the interactions of extracellular ligands and their corresponding cell-surface receptors (1). The initial studies relied on laborious, vacuum-filtration techniques that are cumbersome and difficult to automate. Despite these limitations, filtration-based radioligand binding assays were adequate to probe the kinetics of receptor-ligand interactions and to screen small libraries of compounds in search of receptor-binding antagonists. A number of reviews are available that described this methodology in detail (2,3). [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index