| Autor: |
Walker, John M., Taylor, D. Lansing, Haskins, Jeffrey R., Giuliano, Kenneth A., Moore, K. Gregory, Speckmann, Wayne, Herzig, Ronald P. |
| Zdroj: |
High Content Screening; 2006, p245-251, 7p |
| Abstrakt: |
Cellular immunofluorescence studies can be validated by using either specific small interfering RNA (siRNA) duplexes or expression plasmids that induce the expression of specific siRNAs. The usage of either siRNA tool reduces the expression of the specific protein being studied, thus reducing substantially or abolishing the immunofluorescence detected when using a fluorescent antibody that recognizes the protein. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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