| Autor: |
Walker, John M., Schönthal, Axel H., Hassepass, Ingo, Hoffmann, Ingrid |
| Zdroj: |
Checkpoint Controls & Cancer (978-1-58829-500-2); 2004, p153-162, 10p |
| Abstrakt: |
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using fluorescein diphosphate (FDP) as a substrate is recommended. To analyze endogenous Cdc25 phosphatase activities of immuno-precipitates from total cellular extracts, the physiological substrate Cdk1/cyclin B1 is most sensitive. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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