| Autor: |
Walker, John M., O'Brien, Philippa M., Aitken, Robert, Iba, Yoshitaka, Miyazaki, Chie, Kurosawa, Yoshikazu |
| Zdroj: |
Antibody Phage Display; 2002, p259-267, 9p |
| Abstrakt: |
A two-step strategy for changing the specificity of antibodies (Abs) is presented, which we have used to change the specificity of an Ab from 11-deoxycortisol (11-DOC) to cortisol (CS). Two kinds of in vitro mutagenesis are utilized in this protocol: first, mutations are introduced at restricted positions in the complementarity-determining regions (CDRs) by site-directed mutagenesis; second, mutations are introduced into the entire V-coding regions by random mutagenesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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