| Autor: |
Janson, Christopher G., During, Matthew J., Wengel, Jesper, Petersen, Michael, Frieden, Miriam, Koch, Troels |
| Zdroj: |
Peptide Nucleic Acids, Morpholinos & Related Antisense Biomolecules; 2006, p114-132, 19p |
| Abstrakt: |
Preparation of LNA nucleosides requires a number of synthetic steps but very efficient procedures have been developed, as have protocols for synthesis of LNA oligonucleotides on automated DNA synthesizers. In all cases, LNA oligonucloetides have exhibited good aqueous solubility as would be expected from their close structural resemblance to the natural nucleic acids. The universality of LNA mediated high-affinity and specific hybridization has been demonstrated extensively with a large number of duplex forming LNA-oligonucloetides. Most importantly, most of the members of the LNA molecular family have been shown to exert their substantial affinity increase in combination with standard DNA, RNA and contemporary analogues whether inserted as single nucleosides in an oligonucleotide or as blocks of contiguous nucleotides. The works on TFO's is expanding the usefulness of LNA to double strand recognition and it has been demonstrated that LNA it is a promising structure for further base modifications in the pursuit of global sequence specific recognition of DNA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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