| Autor: |
Walker, John M., Molnar, Peter, Hickman, James J., Antoni, C., Ginneken, G. van, Fijnvandraat, Arnoud C. |
| Zdroj: |
Patch-Clamp Methods & Protocols; 2007, p211-217, 7p |
| Abstrakt: |
The method described here to differentiate mouse embryonic stem (ES) cells into cardiomyocytes is adapted from Maltsev et al. and results in a high percentage of spontaneously beating cardiomyocyte-like cells. In order to determine to what extent the differentiating ES cells resemble true cardiomyocytes, the cells were electrophysiologically characterized during differentiation, using the whole-cell variant of the patch-clamp technique. Action potentials (APs) and membrane currents were recorded and analyzed off-line to determine electrophysiological changes during development. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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