| Autor: |
Walker, John M., Choo, K. H. Andy, Raap, Anton K., van de Rijke, Frans M., Dirks, Roeland W. |
| Zdroj: |
In Situ Hybridization Protocols; 2000, p293-300, 8p |
| Abstrakt: |
In basic and applied biomedical research there is a considerable interest in having reliable RNA in situ hybridization techniques, since these allow the detection of RNA expression in a morphological context at the individual cell level. Conventional molecular biology techniques for RNA analysis such as Northern hybridizations, RNase protection assays, and reverse transcription polymerase chain reactions (RT-PCR) demand the homogenization of the tissue or cell sample. In these cases the possibility of relating (changes in) RNA expression to histo-and cytomorphological information is lost to a very large extent. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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