| Autor: |
Walker, John M., Hall, Walter A., Pennell, Christopher A., Pauza, Mary E. |
| Zdroj: |
Immunotoxin Methods & Protocols; 2001, p17-29, 13p |
| Abstrakt: |
This chapter summarizes our experience with the design and expression of single-chain Fv (sFv) immunotoxins (sFv-IT) specific for CD7, a differentiation antigen found on human peripheral T and NK cells, and on subsets of committed myeloid and lymphoid progenitors in the bone marrow (1-1). In vitro studies have shown that CD7 participates in the activation and adhesion of mature T and NK cells, but the exact role it plays in the development and function of these cells in vivo remains obscure (5, 6). A recent analysis of CD7-deficient mice revealed an association between resistance to endotoxin-induced shock and selective deficiencies of liver NK1.1+/CD3+ T-cell numbers and serum interferon-γ (IFN-γ) levels (7). These data suggest that CD7 participates in the development or migration of murine NK1.1+ T-cells in the liver, and that reduced numbers of these cells results in decreased cytokine production elicited by bacterial lipopolysaccharides. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
