| Autor: |
Giardi, Maria Teresa, Piletska, Elena V., Johanningmeier, Udo, Bertalan, Ivo, Hilbig, Lydia, Schulze, Jana, Wilski, Stefan, Zeidler, Edda, Oettmeier, Walter |
| Zdroj: |
Biotechnological Applications of Photosynthetic Proteins: Biochips, Biosensors & Biodevices; 2006, p46-56, 11p |
| Abstrakt: |
Photosystem II (PSII) is a light driven machine, which supplies our atmosphere with oxygen and, if properly engineered, can be developed into a specific sensor for various pollutants. Its reaction center subunit D1 has long been a target for genetic engineering. It is known to bind a variety of herbicides in a pocket which is naturally occupied by the plastoquinone molecule QB. Amino acid residues lining this binding niche provide ligands to a diverse set of inhibitors, which can loose or gain affinity upon substitution of certain amino acids. One can exploit this and other properties of the D1 protein by changing the corresponding psbA gene which is located on the chloroplast genome in algae and higher plants. We have developed fast site-specific and random mutagenesis techniques specifically adjusted for psbA-gene manipulation in the unicellular green alga Chlamydomonas reinhardtii. Using these protocols mutant collections were generated which have the innate potential of becoming an array of sensitive and specific biosensors. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
