| Autor: |
Walker, John M., Hall, Robert D., Grout, Brian W. W. |
| Zdroj: |
Plant Cell Culture Protocols; 1999, p115-125, 11p |
| Abstrakt: |
The essence of meristem-tip culture is the excision of the organized apex of the shoot from a selected donor plant for subsequent in vitro culture. The conditions of culture are regulated to allow only for organized outgrowth of the apex directly into a shoot, without the intervention of any adventitious organs (1-3). The excised meristem tip is typically small (often <1 mm in length) and is removed by sterile dissection under the microscope, as in the potato example detailed in this chapter (Fig. 1). The explant comprises the apical dome and a limited number of the youngest leaf primordia, and excludes any differentiated provascular or vascular tissues. A major advantage of working with such a small explant is the potential that this holds for excluding pathogenic organisms that may have been present in the donor plants from the in vitro culture (see below). A second advantage is the genetic stability inherent in the technique, since plantlet production is from an already differentiated apical meristem and propagation from adventitious meristems can be avoided (3-9). Shoot development directly from the meristem avoids callus tissue formation and adventitious organogenesis, ensuring that genetic instability and somaclonal variation are minimized. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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