| Autor: |
Walker, John M., Griffin, Hugh G., Griffin, Annette M., Hall, Len |
| Zdroj: |
DNA Sequencing Protocols; 1993, p357-372, 16p |
| Abstrakt: |
The Du Pont Genesis™ 2000 DNA Analysis System consists of an instrument, reagents capable of labeling DNA with novel fluorescent dideoxynucleotides (1), and a Macintosh® II computer to operate the instrument and perform all subsequent data analysis The instrument houses a vertical electrophoresis apparatus (similar to a standard manual sequencing setup) with associated buffer reservoirs and high voltage power supply, together with a fluorescence detection system An argon laser sweeps across the 12-lane sequencing gel and excites fluorescently labeled DNA fragments as they electrophorese past a fixed window toward the bottom of the gel, and the emitted fluorescence is detected simultaneously by two photomultiplier tubes with differing filters (Fig 1), and a Macintosh® II computer to operate the instrument and perform all subsequent data analysis. The instrument houses a vertical electrophoresis apparatus (similar to a standard manual sequencing setup) with associated buffer reservoirs and high voltage power supply, together with a fluorescence detection system. An argon laser sweeps across the 12-lane sequencing gel and excites fluorescently labeled DNA fragments as they electrophorese past a fixed window toward the bottom of the gel, and the emitted fluorescence is detected simultaneously by two photomultiplier tubes with differing filters (Fig. 1) [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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