Electrophoresis of Sequence Reaction Samples.

Autor: Walker, John M., Griffin, Hugh G., Griffin, Annette M., Bankier, Alan T.
Zdroj: DNA Sequencing Protocols; 1993, p121-130, 10p
Abstrakt: The underlying principle of both main DNA sequencing methods by Sanger (1) and Maxam and Gilbert (2), is the ability to fractionate and resolve long, single-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (3). [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index