| Autor: |
Kusano, Mitsuo, Shioda, Seiji, Suzuki, Hiroshi, Iso, Yoshitaka, Kusuyama, Taro, Omori, Yasutoshi, Soda, Teruko, Sato, Takatoshi, Tsunoda, Fumiyoshi, Shoji, Makoto, Koba, Shinji, Geshi, Eiichi, Katagiri, Takashi, Tomoyasu, Shigeru |
| Zdroj: |
New Frontiers in Regenerative Medicine; 2007, p57-65, 9p |
| Abstrakt: |
[Background] Efficacy of autologous bone marrow-mononuclear cell (BM-MNC) implantation as therapeutic angiogenesis has been reported in patients with severe peripheral artery disease. In addition to containing CD34 positive-cells, sorted BM-MNCs contain an abundance of CD34-negative cells. No studies have yet elucidated which types of CD34-negative cells influence the clinical appearance in BM-MNC implantation. We investigated the correlations of morphologically classified cell types of sorted BM-MNCs with changes in the ankle brachial index (ABI) and transcutaneous oxygen pressure (TcO2). [Material and Methods] Seven patients with severe peripheral arterial disease who were not candidates for angioplasty or surgical operation underwent BM-MNC implantation. The sorted BM-MNCs using a cell separator were classified on the basis of May-Giemsa staining, and CD34-positive cells were counted. ABI and TcO2 were performed before and after BM-MNC implantation. [Results] Mean ABI (p<0.05) and mean TcO2 (p<0.005) from baseline to 4 weeks after the implantation were significantly increased. The numbers of total injected cells and CD34-positive cells were not correlated with Δ TcO2 from before to 4 weeks. Among the cell types analyzed, ΔTcO2 showed significant negative correlations with the percentage of mature neutrophils (p<0.01) and significant positive correlations with the percentage of erythroblasts (p<0.05). [Conclusions] Neutrophils could be an inverse regulator and erythroblasts could be a positive regulator in clinical BM-MNC implantation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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