| Autor: |
Ferretti, Claudio, Mauri, Giancarlo, Zandron, Claudio, Junghuei Chen, Deaton, Russell, Garzon, Max H., Jin Woo Kim, Wood, David, Hong Bi, Carpenter, Dylan, Yu-Zhen Wang |
| Zdroj: |
DNA Computing; 2005, p50-61, 12p |
| Abstrakt: |
Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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