| Autor: |
Zhi Yong Wang, Cai-Huan Ke, Yi-Lei Wang, Zhi-Qun Xiao, Ka Chai Ho, Ka Hou Chu |
| Zdroj: |
Journal of Shellfish Research; Dec2007, Vol. 23 Issue 4, p1147-1151, 5p, 2 Diagrams, 2 Charts |
| Abstrakt: |
Amplified fragment length polymorphism (AFLP) technology was used to reveal genetic variations and divergence of Haliotis discus and H. diversicolar. the two economically important abalone species in China. Three sets of selective primers were used for amplification, and 350 bands ranging in size from 80 bp to about 500 bp were scored. The mean percentages of polymorphic bands in the four taxa varied from 51.7% to 62.7%. Similarity indices and genetic distances between individuals of the same species were greater than 0.8 and smaller than 0.2, respectively, whereas the values among individuals of different species were smaller than 0.3 and greater than 1.2, respectively. AFLP profiles of the two abalone species were distinct and could be used fur species identification. Specific AFLP bands were also identified for discriminating the two subspecies of H. discus. Yet no diagnostic bands were found to differentiate the two subspecies of H. diversicolar. The lack of genetic divergence between these two subspecies supports results from a parallel study based on nuclear and mitochondrial DNA sequence analyses. We suggest that the subspecies status of H. diversicolar diversicolar and H. diversicolar supertexta is questionable. Results of this study are applicable to resource management and future genetic improvement of the two abalone species in China. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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