| Autor: |
Admyre, Charlotte, Bohle, Barbara, Johansson, Sara M., Focke-Tejkl, Margarete, Valenta, Rudolf, Scheynius, Annika, Gabrielsson, Susanne |
| Předmět: |
|
| Zdroj: |
Journal of Allergy & Clinical Immunology; Dec2007, Vol. 120 Issue 6, p1418-1424, 7p |
| Abstrakt: |
Background: Exosomes are vesicles of 30 to 100 nm produced by inward budding of endosomal compartments and are released by a range of different cell types. Exosomes from antigen-presenting cells carry immunorelevant molecules like MHC class I and II and costimulatory molecules and thus are suggested to have a role in immune modulation. Objective: To investigate the role of antigen-presenting cell derived exosomes in allergen presentation and T-cell stimulation. Methods: Exosomes were isolated from supernatants of B-cell lines derived from patients with birch pollen allergy. The exosomes were characterized with regard to the expression of surface molecules by flow cytometry. Moreover, exosomes were loaded with T-cell–activating peptides from the major birch allergen Bet v 1, and binding was tested with ELISA. Loaded exosomes were used for stimulation of Bet v 1–specific T-cell lines. Cell proliferation and cytokine production were assessed. Results: The exosomes had a phenotype typical of B cell–derived exosomes with expression of MHC, costimulatory molecules like CD86, tetraspanin proteins such as CD81, and CD19. Furthermore, B cell–derived exosomes bound Bet v 1–derived peptides and subsequently induced a dose-dependent T-cell proliferation. In addition to proliferation, T cells synthesized the cytokines IL-5 and IL-13 in response to peptide-loaded exosomes. Conclusion: These results demonstrate for the first time that exosomes isolated from B cells can present allergen-derived peptides and thereby induce T-cell proliferation and TH2-like cytokine production. Clinical implications: Our data suggest that exosomes from B lymphocytes are an immunostimulatory factor in allergic immune responses. [Copyright &y& Elsevier] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect