Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine.

Autor: DeFlaun, M.F., Fredrickson, J.K, Dong, H., Pfiffner, S.M., Onstott, T.C., Balkwill, D.L., Streger, S.H., Stackebrandt, E., Knoessen, S., van Heerden, E.
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Zdroj: Systematic & Applied Microbiology; Mar2007, Vol. 30 Issue 2, p152-164, 13p
Abstrakt: Abstract: A thermophilic facultative bacterial isolate was recovered from 3.2km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50°C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65°C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4–6μm long×0.5μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans. [Copyright &y& Elsevier]
Databáze: Supplemental Index