| Autor: |
Koulen, Peter, Duncan, R. Scott, Liu, Jiyuan, Cohen, Nancy E., Yannazzo, Jo-Ann S., McClung, Nathalie, Lockhart, Courtney L., Branden, Michael, Buechner, Matthew |
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| Zdroj: |
Cell Calcium; Jun2005, Vol. 37 Issue 6, p593-601, 9p |
| Abstrakt: |
Abstract: Polycystin-2, a member of the TRP family of calcium channels, is encoded by the human PKD2 gene. Mutations in that gene can lead to swelling of nephrons into the fluid-filled cysts of polycystic kidney disease. In addition to expression in tubular epithelial cells, human polycystin-2 is found in muscle and neuronal cells, but its cell biological function has been unclear. A homologue in Caenorhabditis elegans is necessary for male mating behavior. We compared the behavior, calcium signaling mechanisms, and electrophysiology of wild-type and pkd-2 knockout C. elegans. In addition to characterizing PKD-2-mediated aggregation and mating behaviors, we found that polycystin-2 is an intracellular Ca2+ release channel that is required for the normal pattern of Ca2+ responses involving IP3 and ryanodine receptor-mediated Ca2+ release from intracellular stores. Activity of polycystin-2 creates brief cytosolic Ca2+ transients with increased amplitude and decreased duration. Polycystin-2, along with the IP3 and ryanodine receptors, acts as a major calcium-release channel in the endoplasmic reticulum in cells where rapid calcium signaling is required, and polycystin-2 activity is essential in those excitable cells for rapid responses to stimuli. [Copyright &y& Elsevier] |
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