| Autor: |
Dela, Agnieszka, Sajduda, Anna, Pawłowska, Irena, Dziadek, JarosŁaw |
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| Zdroj: |
Journal of Infection; May2006, Vol. 52 Issue 5, p346-353, 8p |
| Abstrakt: |
Summary: Objectives: The aim of the present study was to characterize Mycobacterium tuberculosis strains isolated in the area of Łódź, Poland, from 1996 to 2000. Methods: Two hundred sixty three isolates from 250 patients with tuberculosis were analysed by IS6110 restriction fragment length polymorphism (RFLP) and the double-repetitive-element PCR (DRE-PCR) method when indicated. Results: The isolates were found to show a great heterogeneity and only 52 strains (20.8%) occurred in 20 clusters of 2–5 identical clones. Despite this diversity of IS6110 RFLP patterns, a computer analysis of similarities revealed a high level of relatedness (at least 90%) among 38.4% of different patterns. Most of the patients with clustered strains showed no apparent epidemiologic links with other patients whose strains had the same pattern. Utilisation of the DRE-PCR analysis as an additional typing test allowed to differentiate M. tuberculosis strains with a discriminating capacity similar to that of the IS6110 RFLP. Also, DRE-PCR differentiated nine strains that were indistinguishable by the RFLP analysis. Conclusions: Both methods used for the molecular characterization of M. tuberculosis clinical isolates showed similar discriminating ability. DRE-PCR analysis proved a simple, rapid and cost-effective adjunct to the IS6110 RFLP reference method. It could be applied as a screening test, thus, reducing the number of isolates that need further subtyping with the IS6110 RFLP to those initially clustered. [Copyright &y& Elsevier] |
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