| Autor: |
Khan, Md. Iqbal Hassan, Ito, Kousuke, Hyeung Kim, Hiroyuki Ashida, Ishikawa, Takahiro, Shibata, Hitoshi, Sawa, Yoshihiro |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Oct2005, Vol. 69 Issue 10, p1861-1870, 10p, 2 Diagrams, 3 Charts, 7 Graphs |
| Abstrakt: |
The article examines the molecular properties and enhancement of the thermostability of glutamate dehydrogenase from Bacillus subtilis by random mutagenesis. An error-prone polymerase chain reaction was performed to improve the stability of the enzyme. Two single mutant enzymes, Q144R and E27F were isolated from the final mutant library. It has been discovered that Q144 can be used as a template gene to modify the substrate specificity of Bs-GluDH for industrial use. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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