| Abstrakt: |
The importance of angiogenesis lies in its participation as a crucial physiological process for wound healing and embryonic development, but it is also involved in pathological processes. Various types of experimental approaches are used to study angiogenesis, including in vitro, in vivo, and ex vivo methods. However, ethical and technical challenges persist in its execution. Standardize an ex vivo model with a 3D fibrin matrix from fragments of oral reactive lesions. Previous informed consent, biological material of oral reactive lesions was acquired, under aseptic conditions. The tissue was sectioned to obtain 1 mm3 fragments, which was embedded in a fibrin gel with VEGF induction followed by microscopic visualization, and confirmation of protovascular projections by immunofluorescence for endothelial markers was performed. Quantification of vascular density and length was carried out, descriptive analysis was conducted. Protovascular structures can be induced (in 72 hours), in some cases, the sprout acquired an initial pseudotubular conformation, whereas in others it originated from a precursor mantle, observing that lesions with a greater fibrous component had a better interaction with the angiogenic model. The length and branches were developed in a time-dependent manner, induction was also demonstrated in samples subjected to cryopreservation treatment, which had never been reported before. Ex vivo models, particularly with the use of human tissue considered waste from excisional biopsy treatments, aim to close the gap in the study of angiogenesis. These methods need to be refined to overcome limitations and achieve more reliable results. [ABSTRACT FROM AUTHOR] |
Full Text from ScienceDirect