| Autor: |
Zhao, Qing, Duck, Lennard Wayne, Killian, John T., Rosenberg, Alexander F., Mannon, Peter J., King, R. Glenn, Denson, Lee A., Kugathasan, Subra, Janoff, Edward N., Jenmalm, Maria C., Elson, Charles O. |
| Zdroj: |
Gastroenterology (0016-5085); Dec2024, Vol. 167 Issue 7, p1415-1428, 14p |
| Abstrakt: |
Inflammatory bowel disease (IBD) is a chronic manifestation of dysregulated immune response to the gut microbiota in genetically predisposed hosts. Nearly half of patients with Crohn's disease (CD) develop selective serum immunoglobulin (Ig)G response to flagellin proteins expressed by bacteria in the Lachnospiraceae family. This study aimed to identify the binding epitopes of these IgG antibodies and assess their relevance in CD and in homeostasis. Sera from an adult CD cohort, a treatment-naïve pediatric CD cohort, and 3 independent non-IBD infant cohorts were analyzed using novel techniques including a flagellin peptide microarray and a flagellin peptide cytometric bead array. A dominant B cell peptide epitope in patients with CD was identified, located in the highly conserved "hinge region" between the D0 and D1 domains at the amino-terminus of Lachnospiraceae flagellins. Elevated serum IgG reactivity to the hinge peptide was strongly associated with incidence of CD and the development of disease complications in children with CD up to 5 years in advance. Notably, high levels of serum IgG to the hinge epitope were also found in most infants from 3 different geographic regions (Uganda, Sweden, and the United States) at 1 year of age, which decrements rapidly afterward. These findings identified a distinct subset of patients with CD, united by a shared reactivity to a dominant commensal bacterial flagellin epitope, that may represent failure of a homeostatic response to the gut microbiota beginning in infancy. [Display omitted] Patients with Crohn's disease and healthy infants share a dominant immunoglobulin G antibody response to the "hinge region" of Lachnospiraceae flagellins, suggesting that failure of establishing homeostasis with the gut microbiota in infancy may contribute to the development of Crohn's disease later in life. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
