| Autor: |
Dani, Sophie, Duin, Sarah, Schütz, Kathleen, Windisch, Johannes, Bernhardt, Anne, Ludwig, Barbara, Gelinsky, Michael, Lode, Anja |
| Zdroj: |
Procedia CIRP; 2024, Vol. 125, p325-330, 6p |
| Abstrakt: |
The co-cultivation of mammalian cells with microalgae is an innovative concept to overcome one major limitation in tissue engineering and regenerative medicine: shortage of oxygen. Under illumination, photosynthetically active microalgae can produce oxygen, consequently increasing the potential construct dimension. In such co-cultures, mammalian cells are exposed to light, which does not reflect their natural conditions and can negatively influence their viability and functionality. In this study, 3D extrusion bioprinted pancreatic murine Islets of Langerhans should be co-cultivated with the bioprinted green microalgae species Scenedesmus sp. to improve the oxygen supply of the Islets in a potential insulin-producing implant. After defining the microalgae partner and a suitable co-culture medium that supported the functionality of both cell types, the focus was put on the effects of long-term red, blue and white light illumination on this system. First, the direct influence of light of different wavelengths on both microalgae and different mammalian cell types was investigated to find an ideal illumination regime, followed by an investigation into the influence of light on the culture medium. It was found that long-term exposure to blue or white light is detrimental to mammalian cells itself as well as the medium, while long-wave light has no effect on the function of mammalian cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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