| Autor: |
Kuo, James, Uzunovic, Jasmina, Jacobson, Amanda, Dourado, Michelle, Gierke, Sarah, Rajendram, Manohary, Keilberg, Daniela, Mar, Jordan, Stekol, Emily, Curry, Joanna, Verstraete, Sofia, Lund, Jessica, Liang, Yuxin, Tamburini, Fiona B, Omattage, Natalie S, Masureel, Matthieu, Rutherford, Steven T, Hackos, David H, Tan, Man-Wah, Byrd, Allyson L |
| Zdroj: |
Journal of Crohn's & Colitis; Jul2024, Vol. 18 Issue 7, p985-1001, 17p |
| Abstrakt: |
Background and Aims This study aimed to identify microbial drivers of inflammatory bowel disease [IBD], by investigating mucosal-associated bacteria and their detrimental products in IBD patients. Methods We directly cultured bacterial communities from mucosal biopsies from paediatric gastrointestinal patients and examined for pathogenicity-associated traits. Upon identifying Clostridium perfringens as toxigenic bacteria present in mucosal biopsies, we isolated strains and further characterized toxicity and prevalence. Results Mucosal biopsy microbial composition differed from corresponding stool samples. C. perfringens was present in eight of nine patients' mucosal biopsies, correlating with haemolytic activity, but was not present in all corresponding stool samples. Large IBD datasets showed higher C. perfringens prevalence in stool samples of IBD adults [18.7–27.1%] versus healthy controls [5.1%]. In vitro , C. perfringens supernatants were toxic to cell types beneath the intestinal epithelial barrier, including endothelial cells, neuroblasts, and neutrophils, while the impact on epithelial cells was less pronounced, suggesting C. perfringens may be particularly damaging when barrier integrity is compromised. Further characterization using purified toxins and genetic insertion mutants confirmed perfringolysin O [PFO] toxin was sufficient for toxicity. Toxin RNA signatures were found in the original patient biopsies by PCR, suggesting intestinal production. C. perfringens supernatants also induced activation of neuroblast and dorsal root ganglion neurons in vitro , suggesting C. perfringens in inflamed mucosal tissue may directly contribute to abdominal pain, a frequent IBD symptom. Conclusions Gastrointestinal carriage of certain toxigenic C. perfringens may have an important pathogenic impact on IBD patients. These findings support routine monitoring of C. perfringens and PFO toxins and potential treatment in patients. [ABSTRACT FROM AUTHOR] |
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