INFLUENCE OF AXL RECEPTOR IN ORAL SQUAMOUS CELL CARCINOMA (OSCC) AND ON THE ASSOCIATED PHENOTYPE OF MYELOID CELLS.

Autor: NASCIMENTO, Camyla Rodrigues, PELEGRIN, Álvaro Formoso, REIS, Ianny Brum, de CARVALHO, Milena Moraes, FERRAREZI, Danilo Paschoal, RODRIGUES, Aldenora Maria Ximenes, ROSSA JR, Carlos
Zdroj: Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology; Jun2024, Vol. 137 Issue 6, pe288-e288, 1p
Abstrakt: AXL is a receptor tyrosine kinase that is overexpressed and considered an oncogene in various types of solid tumors. The objective is to determine the influence of the AXL receptor on OSCC and the associated immune response. A syngeneic orthotopic model of OSCC was induced by injecting 5x10^5 MOC2 cells directly into the floor of the mouth of WT and AXL KO mice. Animals were euthanized after two weeks when 20% of the initial body weight was lost and the tumors dissected, weighted, and measured. Spleens were collected and dissociated for flow cytometry analyses of the phenotype of myeloid cells. Splenocytes were stained with antibodies for CD11b, F4/80, CD80, MHC-II, and MertK. There was no significant difference in tumor size or weight between WT and AXL KO mice. There was a trend of increased numbers of F4/80 macrophages and a significant (p=0.03) increase in CD11b+/MHCII+ cells in AXL KO mice. No difference in CD11b+CD80+ or CD11b+MertK+ cells between WT and AXL KO mice. Lack of AXL receptor did not affect experimental OSCC tumor growth. There is a shift to an M2-like phenotype of myeloid cells in AXL KO mice. Financial Support: FAPESP #2022/01487-0; #2020/00394-2. [ABSTRACT FROM AUTHOR]
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