EFFECTS OF RADIOTHERAPY ON THE MORPHOLOGICAL AND COMPOSITIONAL STRUCTURE OF INTRA-RADICULAR DENTIN: IN VITRO STUDY.

Autor: GONDAK, Rogério, MARTINI, Georgia Ribeiro, MINAMISAKO, Mariana Comparotto, BORDIGNON, Natalia Cristina Trentin, RODRIGUES, Paulo Marcelo, de ALBUQUERQUE JUNIOR, Ricardo Luiz Cavalcanti, BORTOLUZZI, Eduardo Antunes
Zdroj: Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology; Jun2024, Vol. 137 Issue 6, pe273-e273, 1p
Abstrakt: To evaluate the effects of radiotherapy (RDT) on root dentin. Thirty human canines were selected from a biobank and divided into 2 groups. The samples were sectioned in buccolingual direction, and a hemisection was used to evaluate the intrarradicular dentin using scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). The analysis of the dentinal tubules' obliteration was performed with a magnification at 2500x in low vacuum SEM, and the inorganic composition in EDS. The RDT protocol was 70Gy divided into daily doses of 2Gy, for 7 weeks. After the RDT protocol, a new evaluation was performed with the same methodology. Collagen integrity analysis of all samples was performed using Masson's trichrome and picrosirius red staining polarization microscopy. RDT promotes the obliteration of dentinal tubules (p < 0.001), reduction in the composition of calcium (p = 0.012), phosphorus (p = 0.001), magnesium (p < 0.001), and impacts on the integrity of collagen fibers (p < 0.05). RDT affects the structure of root dentin and may interfere with dental procedures. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index