| Autor: |
Daou, Farah, Hafdaran, Rana Zarean, Masante, Beatrice, Gabetti, Stefano, Putame, Giovanni, Zenobi, Eleonora, Mochi, Federico, Bignardi, Cristina, Dell’Atti, Federica, Favero, Francesco, Gaudio, Costantino Del, Massai, Diana, Cochis, Andrea, Rimondini, Lia |
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| Zdroj: |
Chemical Industry / Hemijska Industrija; 2024 Supplement, Vol. 78, p10-10, 1p |
| Abstrakt: |
INTRODUCTION: Perfusion bioreactors are currently a good tool for applications in regenerative medicine and bone tissue engineering. In the realm of bone and cartilage regeneration, pulsed electromagnetic field (PEMF) stimulation has become widely utilized in clinical practice. Numerous signaling pathways involved in its osteogenic, chondrogenic, and anti-inflammatory effects have been identified, but the majority of the pathways are yet unknown. Using a novel in vitro investigation platform, this study aimed to identify the signaling pathways altered by PEMF by exposing3D bone-like models to physiological-like perfusion and PEMF stimulation. EXPERIMENTAL: Bioreactor -An automated perfusion bioreactor with tunable perfusion (0.006-24 mL/min) and a PEMF generator (1.5 mT, 75 Hz) (Fig. 1A & 1B). Scaffolds - 3D-printed polylactic acid (PLA) scaffolds resembling trabecular bone microarchitecture. Biological evaluations - Scaffolds were seeded with human mesenchymal stem cells (hMSCs) and exposed to perfusion (0.3 mL/min) with (“P”) and without (“D”) PEMF stimulation (4 h/day) for 21 days in basal or osteogenic medium. Samples were evaluated in triplicates (n = 3). Static cultures served as control (“S”). RNA sequencing (RNA-Seq) and real-time qPCR were conducted to detect the signaling pathways elicited by PEMF. RESULTS AND DISCUSSION: In the absence of biochemical, and according to RNA-Seq analysis PEMF stimulation in basal medium addresses the four stages of bone regeneration: inflammatory, fibrovascular, bone formation, and bone remodeling stages, even in the absence of a pathological state (Figure 1C). CONCLUSIONS: The suggested in vitro research platform represents a novel tool for studying bone biology. [ABSTRACT FROM AUTHOR] |
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