| Autor: |
Wiese, Jakob J, Manna, Subhakankha, Kühl, Anja A, Fascì, Alberto, Elezkurtaj, Sefer, Sonnenberg, Elena, Bubeck, Marvin, Atreya, Raja, Becker, Christoph, Weixler, Benjamin, Siegmund, Britta, Patankar, Jay V, Prüß, Magdalena S, Schumann, Michael |
| Zdroj: |
Journal of Crohn's & Colitis; Jan2024, Vol. 18 Issue 1, p121-133, 13p |
| Abstrakt: |
Background and Aims Pain is a cardinal symptom in inflammatory bowel disease [IBD]. An important structure in the transduction of pain signalling is the myenteric plexus [MP]. Nevertheless, IBD-associated infiltration of the MP by immune cells lacks in-depth characterisation. Herein, we decipher intra- and periganglionic immune cell infiltrations in Crohn´s disease [CD] and ulcerative colitis [UC] and provide a comparison with murine models of colitis. Methods Full wall specimens of surgical colon resections served to examine immune cell populations by either conventional immuno-histochemistry or immunofluorescence followed by either bright field or confocal microscopy. Results were compared with equivalent examinations in various murine models of intestinal inflammation. Results Whereas the MP morphology was not significantly altered in IBD, we identified intraganglionic IBD-specific B cell- and monocyte-dominant cell infiltrations in CD. In contrast, UC-MPs were infiltrated by CD8+ T cells and revealed a higher extent of ganglionic cell apoptosis. With regard to the murine models of intestinal inflammation, the chronic dextran sulphate sodium [DSS]-induced colitis model reflected CD [and to a lesser extent UC] best, as it also showed increased monocytic infiltration as well as a modest B cell and CD8+ T cell infiltration. Conclusions In CD, MPs were infiltrated by B cells and monocytes. In UC, mostly CD8+ cytotoxic T cells were found. The chronic DSS-induced colitis in the mouse model reflected best the MP-immune cell infiltrations representative for IBD. [ABSTRACT FROM AUTHOR] |
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