| Autor: |
LIU Xiao-Juan, LIU Xiu, ZHANG Ming-Hu, HAO Ming, NING Shun-Zong, YUAN Zhong-Wei, HUANG Lin, LIU Deng-Cai, ZHANG Lian-Quan |
| Zdroj: |
Acta Agronomica Sinica; 2023, Vol. 49 Issue 12, p3145-3161, 8p |
| Abstrakt: |
The union of unreduced female and male gametes leads to spontaneous chromosome doubling, which is not only an important way of the origin of polyploid species but also an essential tool for enhancing crop doubled haploid breeding efficiency. A major quantitative trait locus QTug.sau-3B responsible for unreduced gametes formation was discovered in tetraploid Triticum turgidum wheat and was further transferred to elite common wheat lines using synthetic hexaploid wheat as a bridge. This objective of this study is to make wheat/wheat F1 hybrids between the elite lines with this gene region and commercial cultivars and to evaluate the efficiency of QTug.sau-3B gene leading to spontaneous chromosome doubling. Wheat/wheat F1 hybrids and their parents were pollinated with the fresh pollen of Imperata cylindrica to produce wheat haploid through chromosome elimination of I. cylindrica. Doubled haploid (DH) were then developed by spontaneous chromosome doubling in haploids because of the union of unreduced female and male gametes. In this experiment, 5 F1 hybrid materials and 3 I. cylindrica materials were used for distant hybridization. 4610 florets were pollinated, 1965 seeds were produced, 244 embryos were obtained, and 50 wheat haploid plants were obtained. In different temperature treatments, the haploid plant numbered H31 set seeds at 25°C/18°C (18 h/6 h) and 25°C/10°C (18 h/6 h), and the self-fertilization rate was 4.35% and 2.41%, respectively. The results of this study provide a reference for the establishment of wheat haploid breeding technology of chromosome elimination based on wheat-I. cylindrica hybridization and automatic chromosome doubling based on unreduced gamete genes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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