| Autor: |
Takeru Shimada, Shiho Minato, Yuto Hasegawa, Koji Miyamoto, Yasumasa Minato, Shenton, Matthew R., Kazunori Okada, Hiroshi Kawaide, Tomonobu Toyomasu |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Jan2024, Vol. 88 Issue 1, p8-15, 8p |
| Abstrakt: |
Gibberellins are diterpenoid phytohormones that regulate plant growth, and are biosynthesized from a diterpene intermediate, ent - kaurene, which is produced from geranylgeranyl diphosphate via ent -copalyl diphosphate (ent -CDP). The successive 2 cyclization reactions are catalyzed by 2 distinct diterpene synthases, ent -CDP synthase (ent -CPS) and ent -kaurene synthase (KS). Various diterpene synthase genes involved in specialized metabolism were likely created through duplication and neofunctionalization of gibberellinbiosynthetic ent -CPS and KS genes in crops. Brachypodium distachyon is a monocotyledonous species that is a model plant in grasses. We herein found 1 ent-CPS gene homolog BdCPS and 4 tandemly arrayed KS-like genes BdKS1, KSL2, KSL3, and KSL4 in the B. distachyon genome, a simpler collection of paralogs than in crops. Phylogenetic and biochemical analyses showed that BdCPS and BdKS1 are responsible for gibberellin biosynthesis. BdKSL2 and BdKSL3 are suggested to be involved in specialized diterpenoid metabolism. Moreover, we restored KS activity of BdKSL2 through amino acid substitution. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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