| Autor: |
Xin Liu, Xiaosa Xu, Jinping Li, Liying Shi, Ying Zeng, Siyuan Tang, Wei Liu, Lujuan Jia, Yuhong Li, Jie Zhang |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Sep2023, Vol. 87 Issue 9, p960-971, 12p |
| Abstrakt: |
Inhibition of extensive osteoclastogenesis and bone resorption is considered a potential therapeutic target for the treatment of osteoporosis. Isobavachalcone (IBC) is derived from the traditional Chinese herb Psoralea corylifolia Linn. We showed that IBC dose-dependently suppressed receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastogenesis in bone marrow monocyte/macrophage (BMMs) and osteoclastic bone-resorption function without cytotoxicity at a dose of no more than 8 μm in vitro. Mechanistically, the results of western blot and quantitative real-time polymerase chain reaction (qRT-PCR) indicated that IBC inhibited the RANKL-induced degradation of IκBα and phosphorylation of nuclear factor kappa B (NF-κB) in BMMs, and subsequently downregulated the expression of osteoclastic-specific genes and osteoclastogenesis-related proteins. TRAP staining and qRT-PCR showed that IBC can inhibit osteoclast differentiation by down-regulating the expression of miR-193-3p on osteoclast differentiation. Overall, our findings suggest that IBC may serve as a promising compound for the treatment of osteoporosis and other metabolic bone diseases. The signaling pathway of IBC inhibiting RANKL-induced osteoclast formation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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