| Autor: |
Hobbs, Chloe, Diviney, Leanne, Teck Wee Boo, Gallagher, David, Gethin, Georgina, O'Gara, James |
| Předmět: |
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| Zdroj: |
Journal of Wound Management; Jul2023, Vol. 24 Issue 2, p400-400, 1/2p |
| Abstrakt: |
Aim: This project evaluated the repurposing of currently licensed antibiotics as combinations for the treatment of biofilms formed by Staphylococcus aureus strains isolated from patients with diabetic foot infections. Method: The biofilm characteristics of S. aureus isolates obtained with consent and ethical approval from patients with diabetic foot infections (DFIs) were characterized using 24 hour biofilm assays followed by treatment with the dispersal agents proteinase K, which degrades protein adhesin-type biofilms and sodium metaperiodate which degrades polysaccharide-type biofilms. The activity of antibiotic combinations for the eradication of 24 hour biofilms grown in 96 well microtiter plates was examined using Resazurin cell viability assays and biofilm colony forming unit (CFU) enumeration. Results / Discussion: DFI S. aureus biofilms were highly antibiotic tolerant, up to 1000-fold higher than the antibiotic concentration that would eradicate planktonic-grown bacteria. The clinical isolates produced both polysaccharide-type and protein-type biofilms. The latter was common among the DFI clinical isolates and these biofilms were significantly eradicated by a combination of rifampicin and proteinase K. Other antibiotic combinations were found to be largely ineffective. Furthermore the rifampicin-proteinase K was ineffective in the eradication of polysaccharide type biofilms. These data reveal a new combination therapy with the potential to improve DFI treatment options and also highlight how an understanding of the biofilm characteristics of DFI pathogens will be needed to properly evaluate potential new antibiotic therapies. Conclusion: Combinations of rifampicin and the biofilm dispersal enzyme proteinase K may have potential to improve the treatment of DFIs involving S. aureus isolates producing protein-type biofilms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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