| Abstrakt: |
Aim: The mealworm (Tenebrio Molitor) and mealworm oil (MWO) have been reported to affect antioxidant, anticoagulation, antiadipogenic and anti-inflammatory activities. However, since the function of MWO in wound healing is unclear, we investigated the effects of MWO on the wound healing process. Method: Fibroblast cells and endothelial cells were analyzed after MWO treatment in vitro. The wound healing was investigated in vivo using five rats' wounded backs treated with MWO. We investigated the effects of MWO on the proliferation and migration of fibroblasts, the expressions of wound healing-related genes, and the activation of endothelial cells mediated by MWO-stimulated fibroblasts for angiogenesis. Results / Discussion: The mRNA expression levels of collagen-1, alpha-smooth muscle actin, and vascular endothelial growth factor related to wound healing were increased by MWO in fibroblasts. The wound gap of the fibroblasts narrowed by MWO treatment, which means the migration of fibroblast cells was induced by MWO. Migration and tube formation of the endothelial cell was enhanced in fibroblasts treated with MWO, suggesting that MWO induces angiogenesis for wound healing. The intensity and organization of collagen deposition, expression of CD31 as a marker of vascular endothelial cells for angiogenesis, and the length of regenerated epithelium in skin wounds of rats treated with MWO were increased. Conclusion: It confirmed that MWO promotes wound repair of skin in both in vitro and in vivo models. These results demonstrate that MWO might have the potential as a therapeutic agent for the treatment of skin wounds. [ABSTRACT FROM AUTHOR] |
