| Autor: |
Nakamura, Takamichi, Tanaka, Mitsuko, Maruyama, Akihiko, Higashi, Yowsuke, Kurusu, Yasurou |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology & Biochemistry; Dec2004, Vol. 68 Issue 12, p2498-2504, 7p, 1 Diagram, 9 Graphs |
| Abstrakt: |
Investigates the role of the sequence diversity of the C-terminal segment of the GroEL equatorial domain using GroEL from psychrophylic, mesophilic, and thermophilic bacteria. Construction of chimeric GroELs to show that the C-terminal segment governs the different active temperatures of GroE from Pseudoalteromomas sp. PS1M3, Escherichia coli, and Thermus sp. TB1; Identification of the amino acid residues of E. coli GroEL by site-directed mutagenesis and surface plasmon resonance; Cold-adaptation of E. coli harboring a psychrophilic groEL gene. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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