| Abstrakt: |
In this study, the advanced backcross population of semi-fertility plant 10 (sfpiO) mutation constructed by rice pollen semi-fertile mutant lsm and indica rice cultivar 93-11 (wild-type, WT) was used as the research subjects. Compared to the WT, there were no significant differences in plant height, leaf length, leaf width, tiller number, pollen number and other agronomic traits, but pollen fertility reduced significantly. Pollen microscopy and scanning electron microscopy (SEM) at the late stage of pollen development showed that starch accumulation in some pollen of sfp10 decreased observably, eventually led to pollen abortion. Physiological indexes related pollen development revealed that the contents of proline and starch in pollen of the sfp10 mutant decreased significantly. Sucrose accumulation generally increased in the upstream tissues (source leaf, sink leaf, and stem) of panicle, but sucrose content decreased significantly in panicle, indicating that transport from sucrose to panicle was affected. Genetic analysis indicated that the sfp10 phenotype was controlled by a pair of recessive nuclear genes. Gene preliminary mapping located the mutant site into a 398 kb interval between RM25389 and RM25404 on rice chromosome 10, which contained 3 genes related to sucrose transport and 1 gene related to starch synthesis. This study laid a foundation for further research on fine mapping, gene function, and regulation mechanism of pollen semi-fertility regulation genes. [ABSTRACT FROM AUTHOR] |
