| Autor: |
Salah, Samer, Naser, Walid, Jaber, Omar, Saleh, Yacob, Mustafa, Rawan, Abuhijlih, Ramiz, Abuhijla, Fawzi, Ismaeel, Taleb, Yaser, Sameer, Sultan, Iyad, Mustafa, Nour, Tbakhi, Abdelghani |
| Zdroj: |
Reports of Practical Oncology & Radiotherapy; 2022, Vol. 27 Issue 5, p759-767, 9p |
| Abstrakt: |
Background: There remains an unmet need to identify molecular biomarkers in ewing sarcoma (ES). We sought to assess the influence of the O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation on response and progression- free survival (PFS) following initiation of irinotecan and temozolomide (IT), PFS following initiation of vincristine, doxorubicin, and cyclophosphamide alternating with ifosfamide and etoposide (VDC-IE), and overall survival (OS). Materials and methods: Data of advanced ES patients, treated with IT were retrospectively collected. patients were required to have progression after prior VDC-IE. MGMT promoter methylation was assessed on non-decalcified Formalin-fixed paraffin embedded (FFPE) tissue using methylation sensitive restriction enzyme-quantitative PCR (MSRE-GPCR). survival was estimated by the Kaplan-Meier method. results: A total of 20 ES patients underwent MGMT promoter methylation testing, and were eligible for analysis. Five patients (25%) had methylated MGMT, whereas the remaining (15; 75%) had unmethylated promoter. Five (25%) had objective response to IT, with no observed difference by promoter methylation (p = 0.76). Median PFS from initiation of IT for methylated vs. unmethylated MGMT patients was 4.9 and 1.2 months, respectively, p = 0.69. Median PFS from date of initiation of VDC-IE was significantly superior in the methylated group; 27.8 vs. 8.6 months, p = 0.034. Median OS was superior but not statistically significant in the methylated group. conclusion: MGMT-promoter methylation did not correlate with clinical activity or outcomes following the IT regimen for advanced ES. however, methylated MGMT predicted significantly superior PFS following initiation of the standard VDC-IE protocol. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
