| Abstrakt: |
Pineapple (Ananas comosus) hump extract is a waste product of the pineapple plant. The extract contains alkaloids, flavonoids, saponins, and bromelain enzymes, all of which can inhibit the growth of bacteria that cause periodontal disease. Aim: To determine the effectiveness of pineapple hump extract against Treponema denticola, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum biofilm growth and adhesion. The bacteria were inoculated into nutrient broth medium and then incubated for 24 hours at 37°C. The antibacterial and antibiofilm effectiveness of a pineapple hump extract were evaluated using diffusion inhibition test and crystal violet biofilm assay. Chlorhexidine (0.2%) served as a positive control, and distilled water served as a negative control. Each bacterium (200 µL) was plated in 96 well-plates, followed by incubation for 24 hours at 37°C to form biofilm. Subsequently, pineapple hump extract was added into biofilm well and incubated for 1,6 and 24h. After staining with crystal violet, the biofilm density was calculated using a microplate reader. The Shapiro-Wilk test was used to determine whether the data had a normal distribution. For data with a normal distribution, a one-way analysis of variance (ANOVA) test was conducted. In all tests, a value of p<0.05 was considered statistically significant. Showed that pineapple hump extract were effective in inhibit the bacterial growth. A one-way analysis of variance (ANOVA) test and post hoc test revealed a significant difference (p < 0.05) between the ability of the different concentrations of pineapple hump extract to inhibit A. actinomycetemcomitans, T. denticola, and F. nucleatum biofilm growth. The pineapple hump extract has been proven to inhibit bacterial growth and eradicate biofilms attachment of periodontal pathogens. The pineapple hump extract has potential as antibacterial and antibiofilm agent to prevent periodontal diseases. However, further studies are still needed to explore this result. [ABSTRACT FROM AUTHOR] |
