PLFA Profiling of Coal Mine Spoil: An Integrated Approach for the Assessment of Ecological Restoration.

Autor: Agrawal, Payal, Maharana, Jitesh Kumar, Patel, Amiya Kumar
Předmět:
Zdroj: Journal of Ecophysiology & Occupational Health; Jun2022, Vol. 22 Issue 2, p77-87, 11p
Abstrakt: Mine spoil generated after math of extensive coal mining activities represents disequilibrated geomorphic system. The pedodiversity including its link with biodiversity and landscape ecology describe the spatial diversity has emerged as functional determinants of ecosystem processes. Being the driving force in mediating soil processes, ecosystem restoration through mine spoil genesis is addressed to determine the shift in microbial communities in coal mine spoil over time. PLFA is independent of culture techniques that involve various molecular markers to determine microbial community structure as well as their discrimination based on their origin. PLFAs are rapidly degraded after cell lyses that are synthesized during microbial growth reliably reflecting the viable microbial communities. The study revealed significant variation with respect to their relative distribution of 51 PLFAs indicating the variations in microbial community composition with Shannon diversity index varies from 1.5265 (OB0) to 2.0139 (OB15) and Pielous evenness index from 0.4110 (OB0) to 0.5260 (OB15). Increasing trend in fungal to bacterial ratio was evident from OB0 (0.055) to OB15 (0.348) with the increase in age of mine spoil that revealed the progress in ecological restoration. Besides, the PCA and RDA analysis discriminate chronosequence coal mine spoil into independent clusters, which revealed the microbial community dynamics influencing the pace and progress of ecological restoration. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index