| Autor: |
Aquino, Iara Gonçalves, Cuadra-Zelaya, Florence Juana Maria, Bizeli, Ana Laura Valença, Teixeira, Isadora Ferrari, Coletta, Ricardo Della, Bastos, Débora Campanella, Graner, Edgard |
| Zdroj: |
Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology; Sep2022, Vol. 134 Issue 3, pe218-e218, 1p |
| Abstrakt: |
Cancer stem cells (CSCs) comprise a subpopulation of tumor cells associated with initiation, progression, and chemoresistance. We identified, isolated, and characterized subpopulations of CSC in SCC-9 ZsGreen (SCC-9 ZsG) and LN1-A cell lines derived from oral squamous cell carcinoma (OSCC). SCC-9 ZsG and LN-1A subpopulations were identified and isolated with antibodies against CD44 and CD326 by flow cytometry. Cell morphology, proliferation, migration, clonogenic assay, and protein expression associated with epithelial-mesenchymal transition were carried out. Six and 7 phenotypes were isolated in SCC-9 ZsG and LN-1A cells in relation to its differential expression, respectively. CD44-low/CD326+ subpopulations showed epithelial phenotype with polygonal morphology and high expression of E-cadherin, whereas CD44+/CD326− showed a fusiform morphology with a high expression of vimentin, N-cadherin, and Slug, suggesting a mesenchymal phenotype. CD44-high/CD326− subpopulations were exclusively identified and isolated in LN-1A cells, also presenting a mesenchymal phenotype. Proliferation, migration, and colony formation were increased in CD44+/CD326− compared to CD44-low/CD326+ and parental cells in SCC-9 ZsG. Our preliminary results showed that CD44-high/CD326− cells were correlated to a more malignant phenotype. Further experiments will be needed to better characterize the populations of CSC in OSCC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect